Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay
نویسندگان
چکیده
A magnetic particle-based enzyme-liked immunosorbent assay (mp-ELISA) has been developed as new an alternative immunoassay for Aflatoxin B1 determination. The method is based on conventional competitive ELISA whereby the anti-Aflatoxin B1 antibody is immobilized on the magnetic particles' surface. The influence of the antibody type as well as antibody immobilization on the magnetic beads surface was investigated in detail. Also, optimum values for the general parameters of the method (e.g. tracer concentration, type of antibody, and incubation time) were established. Finally, a sensitive immunoassay method (mp-ELISA) was performed for Aflatoxin B1 determination at ppt level (LOD = 1 ppt Aflatoxin B1).
منابع مشابه
Fabrication of a Voltammetric Biosensor in Combination with Enzyme-linked Immunosorbent Assay to Detect Aflatoxin M1 in Milks
Background and Objectives: Aflatoxin M1 is one the most important mycotoxin contaminants of milks. Various methods have been described to detect aflatoxins in milks, including chromatography, enzyme-linked immunosorbent assay and colorimetry. Regarding high sensitivity of electrochemical sensor based methods, the aim of the present study was to fabricate an accurate sensitive voltametric biosen...
متن کاملThe Measurement of Aflatoxin B1 in Chilli and Black Peppers of Qaemshahr, Iran
Background & Aims: Aflatoxins are a group of toxic compounds found in most plant products such as pistachio, corn, spices, wheat, and rice. This study was carried out to detect the presence of aflatoxin B1 (AFB1) in samples of spices collected from Qaemshahr in Mazandaran province, Iran. Methods: This was a cross-sectional study on the presence of AFB1 in 18 samples of spices including chilli (...
متن کاملThe Determination of Aflatoxins in Paddy and Milled Fractions of Rice in Guyana: Preliminary Results
A survey was conducted in the five rice-growing regions in Guyana to determine the presence of aflatoxins in multiple fractions of rice in June/October 2015 growing season. The fractions were paddy, steamed paddy, cargo rice, white rice and parboiled rice. Samples were analyzed by High Performance Liquid Chromatography. A subset of the samples was further analyzed by enzyme-linked immunosorbent...
متن کاملSensitive Quantification of Aflatoxin B1 in Animal Feeds, Corn Feed Grain, and Yellow Corn Meal Using Immunomagnetic Bead-Based Recovery and Real-Time Immunoquantitative-PCR
Aflatoxins are considered unavoidable natural mycotoxins encountered in foods, animal feeds, and feed grains. In this study, we demonstrate the application of our recently developed real-time immunoquantitative PCR (RT iq-PCR) assay for sensitive detection and quantification of aflatoxins in poultry feed, two types of dairy feed (1 and 2), horse feed, whole kernel corn feed grains, and retail y...
متن کاملHighly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier
Background: Recently, some new nanobiosensors using different nanoparticles or microarray systems for detection of mycotoxins have been designed . However, rapid, sensitive and early detection of aflatoxicosis would be very helpful to distinguish high-risk persons. Objectives: We report a highly sensitive competitive immunoassay using magnetic/silica core shell as a signal intensifier for the d...
متن کامل